Research Tool
Reconstitution calculator.
Calculate stock concentrations and target volumes for laboratory research peptide work. Enter mg in vial + diluent added, read off the working values.
Research Use
Laboratory context only.
This tool calculates concentrations for in-vitro and laboratory research workflows. It does not compute injection volumes, human doses, or therapeutic regimens. See Research-Use Disclaimer.
Stock concentration
2.50 mg/ml
2.50 µg/µl
For 10 µg
4.0 µl
For 100 µg
40 µl
For 500 µg
200 µl
For 1 mg
0.40 ml
How It Works
Three numbers, one equation.
Step 1
Stock concentration
Divide peptide mass by diluent volume. A 5 mg vial reconstituted in 2 ml gives a stock of 2.5 mg/ml. This is your reference number for every downstream calculation.
Step 2
Target mass
Decide the mass you want per aliquot or assay point. 10 µg, 100 µg, 1 mg — whatever your protocol calls for. The calculator converts it to the volume needed from your stock.
Step 3
Volume per aliquot
Volume = target mass / stock concentration. For 100 µg from a 2.5 mg/ml stock: 0.1 mg ÷ 2.5 mg/ml = 0.04 ml = 40 µl. Transfer with a calibrated pipette.